PlasmidPros make prep easy and fast.
Step 1 Shipping
Send us your samples using the shipping instructions provided.
Step 2 Transformation
We transform your 1µg DNA sample using a concentration of at least 20 ng/µl into the DH5alpha strain of E.coli at 37˚C.
Step 3 Clone Selection
We plate transformed cells on agar plates that contain the antibiotic to which the plasmid confers resistance.
Step 4 Growth/Harvest
Culture grow overnight at 37˚C in secure, controlled-setting shaker. When ready, we harvest them by centrifugation.
Step 5 Lysis/Purification
Harvested cell paste goes through an alkaline lysis event, and is purified.
Step 6 UV Spectroscopy
We resuspend the pure plasmid DNA it in a set volume of either water or 10 mM Tris at a pH 8.0. Our UV spectroscopy reports the concentration and OD 260/280 ratio of the plasmid on the label of the final deliverable vial.
Step 7 Receive Finished Samples
Receive your finished preps, ready to use.
If your samples require extra attention to increase yields, contact our Pros for custom services.